| 言語種別 | 英語 |
|---|---|
| 発行・発表の年月 | 2021/09/15 |
| 形態種別 | 【論文】研究論文（学術雑誌）＜査読あり＞ |
| 査読 | 査読あり |
| 標題 | A MATLAB-based program for three-dimensional quantitative analysis of micronuclei reveals that neuroinflammation induces micronuclei formation in the brain. |
| 執筆形態 | 共著 |
| 掲載誌名 | Scientific Reports |
| 掲載区分 | 国外 |
| 出版社・発行元 | Nature |
| 巻・号・頁 | 11(18360) |
| 総ページ数 | 13 |
| 担当範囲 | I was not in charge of the wet experiments, but rather developed a program to analyze the cell images obtained from the experimental results and was in charge of verifying the experimental results. |
| 担当区分 | 最終著者 , 責任著者 |
| 著者・共著者 | Sarasa Yano, Kaito Akiyama, Rio Tsuchiya, Hikari Kubotani, Tomoki Chiba, Takeshi Nagata & Fuminori Tsuruta |
| 概要 | The micronucleus is known to be a biomarker for genomic instability, which is a hallmark of tumors and aging.
Although micronuclei often emerge in damaged tissues, the quantitative procedure for analyzing micronuclei accurately has been problematic. Here, we introduce a novel MATLAB-based program for quantifying micronuclei (CAMDi: calculating automatic micronuclei distinction) in vitro and in vivo. CAMDi enables us to measure the accurate size of micronuclei from the threedimensional images. Using CAMDi, we revealed a novel link between the emergence of micronuclei and neuroinflammation. We found that inflammatory stimulation does not increase the number of micronuclei in primary neurons. On the other hand, the administration of lipopolysaccharide into mice slightly increases micronuclei formation in neurons of the hippocampus region. These findings demonstrate that neuronal micronuclei formations are induced by an inflammatory response in a noncell-autonomous manner. |
| researchmap用URL | https://www.nature.com/articles/s41598-021-97640-6 |